Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP53

Cell type

Cell type Class
Gonad
Cell type
A2780
Primary Tissue
Ovary
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
cell line
cell line
A2780
chip antibody
Bethyl Laboratories, A300-247A-M
treatment
Nutlin-3A

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked for 10 minutes at room temperature. The samples were sheared using 2 μL MNase (NEB, M0247S) for 10 minutes, then briefly sonicated 4 cycles (30secs on/30secs off) at 4°C. Samples were then centrifuged and the supernatant was used as the input and for the downstream immunoprecipitation. The immunoprecipitation was performed following the manufacture's protocol of the Active Motif Kit, using 4 μL of the p53 antibody (Bethyl Laboratories, A300-247A-M). ChIP-seq library preparation was completed using the NEBNext Ultra II DNA Library Prep (NEB, E7103S) with multiplex oligos for barcoding (NEB, E7335S).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
32002872
Reads aligned (%)
96.1
Duplicates removed (%)
25.5
Number of peaks
2769 (qval < 1E-05)

hg19

Number of total reads
32002872
Reads aligned (%)
95.9
Duplicates removed (%)
25.8
Number of peaks
2768 (qval < 1E-05)

Base call quality data from DBCLS SRA