Cells were cross-linked for 10 minutes at room temperature. The samples were sheared using 2 μL MNase (NEB, M0247S) for 10 minutes, then briefly sonicated 4 cycles (30secs on/30secs off) at 4°C. Samples were then centrifuged and the supernatant was used as the input and for the downstream immunoprecipitation. The immunoprecipitation was performed following the manufacture's protocol of the Active Motif Kit, using 4 μL of the p53 antibody (Bethyl Laboratories, A300-247A-M). ChIP-seq library preparation was completed using the NEBNext Ultra II DNA Library Prep (NEB, E7103S) with multiplex oligos for barcoding (NEB, E7335S).